Publikation

Ciprofloxacin and epirubicin synergistically induce apoptosis in human urothelial cancer cell lines

Wissenschaftlicher Artikel/Review - 01.03.2012

Bereiche
PubMed
DOI
Kontakt

Zitation
Engeler D, Scandella E, Ludewig B, Schmid H. Ciprofloxacin and epirubicin synergistically induce apoptosis in human urothelial cancer cell lines. Urol Int 2012; 88:343-9.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Urol Int 2012; 88
Veröffentlichungsdatum
01.03.2012
eISSN (Online)
1423-0399
Seiten
343-9
Kurzbeschreibung/Zielsetzung

INTRODUCTION
Few published in vitro studies have shown antitumor drug action or possible synergistic effects of fluoroquinolones. To assess the potential role of combination therapy, cytotoxic effects of ciprofloxacin and epirubicin alone and in combination were determined.

MATERIAL AND METHODS
Human urothelial cancer cell lines HT1197 and HT1376 were exposed in vitro for 1 h to different concentrations of epirubicin (0.02-2 mg/ml) and for 72 h to ciprofloxacin (0.004-0.8 mg/ml). Cytotoxicity was determined using the microculture tetrazolium assay and flow cytometry. Synergistic cytotoxic effects were determined by calculating combination indices.

RESULTS
Median effect concentrations of epirubicin for HT1376 and HT1197 cells were as low as 124 and 117 μg/ml, respectively. Ciprofloxacin-treated cells exhibited profound cytotoxic effects at concentrations of 50-100 μg/ml, which is far below the intravesical concentration reached by standard oral application. In addition, a pronounced synergistic effect was found when the two treatments were combined.

CONCLUSIONS
This study provides evidence that ciprofloxacin and epirubicin exhibit synergistic cytotoxic effects in vitro. After confirmatory animal experiments, future clinical studies of adjuvant chemotherapy after transurethral bladder resection may include treatment arms with combinations of fluoroquinolones based on the observed synergistic effects to reduce both side effects and costs.