Publikation

Translating innate response into long-lasting antibody response by the intrinsic antigen-adjuvant properties of papaya mosaic virus

Wissenschaftlicher Artikel/Review - 07.12.2007

Bereiche
PubMed
DOI

Zitation
Acosta-Ramírez E, Leclerc D, Isibasi A, Becker I, Flores-Romo L, Santos-Argumedo L, Cervantes-Barragan L, Manjarrez-Orduño N, Ramírez-Saldaña M, Gil-Cruz C, Pastelin-Palacios R, Majeau N, Pérez-Flores R, López-Macías C. Translating innate response into long-lasting antibody response by the intrinsic antigen-adjuvant properties of papaya mosaic virus. Immunology 2007; 124:186-97.
Art
Wissenschaftlicher Artikel/Review (Englisch)
Zeitschrift
Immunology 2007; 124
Veröffentlichungsdatum
07.12.2007
eISSN (Online)
1365-2567
Seiten
186-97
Kurzbeschreibung/Zielsetzung

Identifying the properties of a molecule involved in the efficient activation of the innate and adaptive immune responses that lead to long-lasting immunity is crucial for vaccine and adjuvant development. Here we show that the papaya mosaic virus (PapMV) is recognized by the immune system as a pathogen-associated molecular pattern (PAMP) and as an antigen in mice (Pamptigen). A single immunization of PapMV without added adjuvant efficiently induced both cellular and specific long-lasting antibody responses. PapMV also efficiently activated innate immune responses, as shown by the induction of lipid raft aggregation, secretion of pro-inflammatory cytokines, up-regulation of co-stimulatory molecules on dendritic cells and macrophages, and long-lasting adjuvant effects upon the specific antibody responses to model antigens. PapMV mixed with Salmonella enterica serovar Typhi (S. typhi) outer membrane protein C increased its protective capacity against challenge with S. typhi, revealing the intrinsic adjuvant properties of PapMV in the induction of immunity. Antigen-presenting cells loaded with PapMV efficiently induced antibody responses in vivo, which may link the innate and adaptive responses observed. PapMV recognition as a Pamptigen might be translated into long-lasting antibody responses and protection observed. These properties could be used in the development of new vaccine platforms.