Publikation
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation
Wissenschaftlicher Artikel/Review - 16.01.2018
Emgård Johanna, Svensson Mattias, Scandella Elke, Hepworth Matthew R, Huber Samuel, Ludewig Burkhard, Peduto Lucie, Villablanca Eduardo J, Veiga-Fernandes Henrique, Pereira João P, Flavell Richard A, Kvedaraite Egle, Melo-Gonzalez Felipe, Kammoun Hana, García-Cassani Bethania, Chesné Julie, Parigi Sara M, Jacob Jean-Marie, Cheng Hung-Wei, Evren Elza, Das Srustidhar, Czarnewski Paulo, Sleiers Natalie, Willinger Tim
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Group 3 innate lymphoid cells (ILC3s) sense environmental signals and are critical for tissue integrity in the intestine. Yet, which signals are sensed and what receptors control ILC3 function remain poorly understood. Here, we show that ILC3s with a lymphoid-tissue-inducer (LTi) phenotype expressed G-protein-coupled receptor 183 (GPR183) and migrated to its oxysterol ligand 7α,25-hydroxycholesterol (7α,25-OHC). In mice lacking Gpr183 or 7α,25-OHC, ILC3s failed to localize to cryptopatches (CPs) and isolated lymphoid follicles (ILFs). Gpr183 deficiency in ILC3s caused a defect in CP and ILF formation in the colon, but not in the small intestine. Localized oxysterol production by fibroblastic stromal cells provided an essential signal for colonic lymphoid tissue development, and inflammation-induced increased oxysterol production caused colitis through GPR183-mediated cell recruitment. Our findings show that GPR183 promotes lymphoid organ development and indicate that oxysterol-GPR183-dependent positioning within tissues controls ILC3 activity and intestinal homeostasis.