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Multi-omic Approach with Genome editing to unravel the Resistance Mechanisms to Ceftazidime Avibactam in Pseudomonas aeruginosa,

Baharak Babouee Flury & Maria Kogadeeva Zimmermann

abstract In this study, a collaborative work with EMBL Heidelberg, ETH- D BSSE Empa (Materials Science and Technology), we deciphered –hitherto unknown- resistance mechanisms of P. aeruginosa to ceftazidime- avibactam (CZA), a novel antibiotic with a multi-omics (genomics, transcriptomics, proteomics) approach. Resistant derivatives were generated by a multistep resistance selection for CZA and meropenem and cross-resistance evaluated. This study is at the stage of genome editing, where we are implementing interesting mutations found in WGS (compared to transcriptome and proteome) into the parental strains.
   
project partner Maria Kogadeeva-Zimmermann, EMBL Heidelberg
Steven Schmitt, ETH D-BSSE
Qun Ren, EMPA
type of project fundamental research
status ongoing - follow up
start of project 2019
end of project 2022
project manager Baharak Babouee Flury