Project

Prevalence and functional role of read-through fusion transcripts in lung cancer

Automatically Closed · 2016 until 2018

Type
Fundamental Research
Range
Monocentric project at KSSG
Units
Status
Automatically Closed
Start Date
2016
End Date
2018
Financing
Others
Brief description/objective

The​ ​ aim​ ​ of​ ​ this​ ​ exploratory​ ​ study​ ​ is​ ​ to​ ​ assess​ ​ the​ ​ prevalence​ ​ and​ ​ the​ ​ functional​ ​ role​ ​ of RTFTs​ ​ in​ ​ lung​ ​ cancer.​ ​ Total​ ​ RNA​ ​ extracted​ ​ from​ ​ lung​ ​ biopsies​ ​ obtained​ ​ in​ ​ 50​ ​ patients diagnosed​ ​ with​ ​ non-small​ ​ cell​ ​ lung​ ​ cancer​ ​ (50​ ​ tumor​ ​ biopsies​ ​ + ​ ​ 50​ ​ matched non-malignant​ ​ lung​ ​ tissue​ ​ adjacent​ ​ to​ ​ tumors)​ ​ as​ ​ well​ ​ as​ ​ in​ ​ 50​ ​ patients​ ​ with inflammatory​ ​ lung​ ​ diseases​ ​ (total:​ ​ 150​ ​ samples)​ ​ will​ ​ be​ ​ profiled​ ​ using​ ​ RNA​ ​ sequencing. A​ ​ new​ ​ algorithm​ ​ for​ ​ the​ ​ identification​ ​ of​ ​ putative​ ​ RTFTs​ ​ will​ ​ be​ ​ applied​ ​ to​ ​ the​ ​ RNA sequencing​ ​ data.​ ​ The​ ​ algorithm​ ​ will​ ​ be​ ​ challenged​ ​ to​ ​ other​ ​ existing​ ​ fusion​ ​ genes identification​ ​ tools.​ ​ The​ ​ findings​ ​ in​ ​ terms​ ​ of​ ​ newly​ ​ discovered​ ​ fusion​ ​ genes​ ​ will​ ​ be validated​ ​ by​ ​ interrogating​ ​ databases​ ​ of​ ​ chimeric​ ​ transcripts​ ​ [7].​ ​ Comparison​ ​ with​ ​ other available​ ​ data​ ​ set​ ​ will​ ​ be​ ​ carried​ ​ out.