Multi-omic Approach with Genome editing to unravel the Resistance Mechanisms to Ceftazidime Avibactam in Pseudomonas aeruginosa,
Baharak Babouee Flury & Maria Kogadeeva Zimmermann
| abstract | In this study, a collaborative work with EMBL Heidelberg, ETH- D BSSE Empa (Materials Science and Technology), we deciphered –hitherto unknown- resistance mechanisms of P. aeruginosa to ceftazidime- avibactam (CZA), a novel antibiotic with a multi-omics (genomics, transcriptomics, proteomics) approach. Resistant derivatives were generated by a multistep resistance selection for CZA and meropenem and cross-resistance evaluated. This study is at the stage of genome editing, where we are implementing interesting mutations found in WGS (compared to transcriptome and proteome) into the parental strains. |
| project partner |
Maria Kogadeeva-Zimmermann, EMBL Heidelberg Steven Schmitt, ETH D-BSSE Qun Ren, EMPA |
| type of project | fundamental research |
| status | completed |
| start of project | 2019 |
| end of project | 2023 |
| project manager | Baharak Babouee Flury |