Kantonsspital St.Gallen

Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity

Alejandro Prados, Lucas Onder, Hung-Wei Cheng, Urs Mörbe, Mechthild Lütge, Cristina Gil Cruz, Christian Perez Shibayama, Vasiliki Koliaraki, Burkhard Ludewig & George Kollias

abstract Fibroblastic reticular cells (FRCs) determine the organization of lymphoid organs and control immune cell interactions. While the cellular and molecular mechanisms underlying FRC differentiation in lymph nodes and the splenic white pulp have been elaborated to some extent, in Peyer's patches (PPs) they remain elusive. Using a combination of single-cell transcriptomics and cell fate mapping in advanced mouse models, we found that PP formation in the mouse embryo is initiated by an expansion of perivascular FRC precursors, followed by FRC differentiation from subepithelial progenitors. Single-cell transcriptomics and cell fate mapping confirmed the convergence of perivascular and subepithelial FRC lineages. Furthermore, lineage-specific loss- and gain-of-function approaches revealed that the two FRC lineages synergistically direct PP organization, maintain intestinal microbiome homeostasis and control anticoronavirus immune responses in the gut. Collectively, this study reveals a distinct mosaic patterning program that generates key stromal cell infrastructures for the control of intestinal immunity.
citation Prados A, Onder L, Cheng H W, Mörbe U, Lütge M, Gil Cruz C, Perez Shibayama C, Koliaraki V, Ludewig B, Kollias G. Fibroblastic reticular cell lineage convergence in Peyer's patches governs intestinal immunity. Nat Immunol 2021; 22:510-519.
type journal paper/review (English)
date of publishing 11-03-2021
journal title Nat Immunol (22/4)
ISSN electronic 1529-2916
pages 510-519
PubMed 33707780
DOI 10.1038/s41590-021-00894-5