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Laser-facilitated epicutaneous immunization of mice with SARS-CoV-2 spike protein induces antibodies inhibiting spike/ACE2 binding

Sandra Scheiblhofer, Stephan Drothler, Werner Braun, Reinhard Braun, Maximilian Bösch & Richard Weiss

abstract The skin represents an attractive target tissue for vaccination against respiratory viruses such as SARS-CoV-2. Laser-facilitated epicutaneous immunization (EPI) has been established as a novel technology to overcome the skin barrier, which combines efficient delivery via micropores with an inherent adjuvant effect due to the release of danger-associated molecular patterns. Here we delivered the S1 subunit of the Spike protein of SARS-CoV-2 to the skin of BALB/c mice via laser-generated micropores with or without CpG-ODN1826 or the B subunit of heat-labile enterotoxin of E.coli (LT-B). EPI induced serum IgG titers of 1:3200 that could be boosted 5 to 10-fold by co-administration of LT-B and CpG, respectively. Sera were able to inhibit binding of the spike protein to its receptor ACE2. Our data indicate that delivery of recombinant spike protein via the skin may represent an alternative route for vaccines against Covid-19.
   
citation Scheiblhofer S, Drothler S, Braun W, Braun R, Bösch M, Weiss R. Laser-facilitated epicutaneous immunization of mice with SARS-CoV-2 spike protein induces antibodies inhibiting spike/ACE2 binding. Vaccine 2021; 39:4399-4403.
   
type journal paper/review (English)
date of publishing 26-06-2021
journal title Vaccine (39/32)
ISSN electronic 1873-2518
pages 4399-4403
PubMed 34226102
DOI 10.1016/j.vaccine.2021.06.068