Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases

Publication

Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases

Journal Paper/Review - Jun 21, 2004

van Swieten Paul F, Ploegh Hidde L, van der Marel Gijsbert A, Driessen Christoph, Leeuwenburgh Michiel A, Kalbacher Hubert, Wong Chung-Sing, Reich Michael, Kessler Benedikt M, van den Nieuwendijk Adrianus M C H, Maehr Rene, Overkleeft Herman S

Citation

van Swieten P, Ploegh H, van der Marel G, Driessen C, Leeuwenburgh M, Kalbacher H, Wong C, Reich M, Kessler B, van den Nieuwendijk A, Maehr R, Overkleeft H. Development of an isotope-coded activity-based probe for the quantitative profiling of cysteine proteases. Bioorganic & medicinal chemistry letters 2004; 14:3131-4.

Type

Journal Paper/Review (English)

Journal

Bioorganic & medicinal chemistry letters 2004; 14

Publication Date

Jun 21, 2004

Issn Print

0960-894X

Pages

3131-4

Brief description/objective

Quantification studies of complex protein mixtures have been restricted mainly to whole cell extracts. Here we describe the synthesis of two sets of isotope-coded activity-based probes that allow quantitative functional proteomics experiments on the cathepsins.