Phenotypic and Genomic Analyses of Burkholderia stabilis Clinical Contamination, Switzerland
Helena M B Seth-Smith, Carlo Casanova, Rami Sommerstein, Dominik M Meinel, Mohamed M H Abdelbary, Dominique S Blanc, Sara Droz, Urs Führer, Reto Lienhard, Claudia Lang, Olivier Dubuis, Matthias Schlegel, Andreas Widmer, Peter M Keller, Jonas Marschall & Adrian Egli
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abstract
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A recent hospital outbreak related to premoistened gloves used to
wash patients exposed the difficulties of defining Burkholderia
species in clinical settings. The outbreak strain displayed key B.
stabilis phenotypes, including the inability to grow at 42°C; we
used whole-genome sequencing to confirm the pathogen was B.
stabilis. The outbreak strain genome comprises 3 chromosomes and a
plasmid, sharing an average nucleotide identity of 98.4% with B.
stabilis ATCC27515 BAA-67, but with 13% novel coding sequences. The
genome lacks identifiable virulence factors and has no apparent
increase in encoded antimicrobial drug resistance, few insertion
sequences, and few pseudogenes, suggesting this outbreak was an
opportunistic infection by an environmental strain not adapted to
human pathogenicity. The diversity among outbreak isolates (22 from
patients and 16 from washing gloves) is only 6 single-nucleotide
polymorphisms, although the genome remains plastic, with large
elements stochastically lost from outbreak isolates.
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citation
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Seth-Smith H M B, Casanova C, Sommerstein R, Meinel D M, Abdelbary M
M H, Blanc D S, Droz S, Führer U, Lienhard R, Lang C, Dubuis O,
Schlegel M, Widmer A, Keller P M, Marschall J, Egli A. Phenotypic
and Genomic Analyses of Burkholderia stabilis Clinical
Contamination, Switzerland. Emerging Infect Dis 2019; 25:1084-1092.
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type
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journal paper/review (English)
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date of publishing
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6-2019
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journal title
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Emerging Infect Dis (25/6)
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ISSN electronic
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1080-6059
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pages
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1084-1092
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PubMed
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31107229
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DOI
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10.3201/eid2506.172119
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