Detection and quantification of new designer drugs in human blood: Part 1 - Synthetic cannabinoids
Julia Ammann, Jenna M McLaren, Dimitri Gerostamoulos & Jochen Beyer
|
abstract
|
Synthetic cannabinoids sprayed on herbal mixtures have been abused
as a new designer drug all over the world since 2004. In 2008, the
first compounds, CP 47,497 and JWH-018, were identified as active
ingredients in these mixtures. Most of the compounds have been
synthesized for research purposes and are potent CB1 and/or CB2
receptor agonists. To investigate the presence of synthetic
cannabinoids in blood samples, a liquid chromatography-tandem mass
spectrometry (LC-MS-MS) method was developed using only 100 µL
of blood. After the addition of 0.2 mL of trizma buffer, the blood
was extracted using liquid-liquid extraction with 1 mL of
1-chlorobutane containing 10% of isopropanol for 5 min on a shaker
at 1,500 rpm. After centrifugation at 12,000 rpm for 1 min, the
separated solvent layer was transferred to an autosampler vial and
evaporated to dryness under N₂. The residue was reconstituted in
methanol and injected into a Shimadzu 8030 LC-MS-MS system to
separate and detect 25 synthetic cannabinoids. The method has been
validated according to international guidelines and was found to be
selective for all tested compounds. Calibration was satisfactory
from 0.5-100 ng/mL, and from 5.0-500 ng/mL. for HU-210, CP 47,497
and the CP 47,497 C-8 homolog, respectively. The extraction
efficiencies ranged from 30-101% and the matrix effects from
67-112%. Accuracy data were within the acceptance interval of
±15% (±20% at the lower limit of quantification) of
the nominal values for all drugs.
|
| |
|
|
citation
|
Ammann J, McLaren J M, Gerostamoulos D, Beyer J. Detection and
quantification of new designer drugs in human blood: Part 1 -
Synthetic cannabinoids. J Anal Toxicol 2012; 36:372-80.
|
| |
|
|
type
|
journal paper/review (English)
|
|
date of publishing
|
16-05-2012
|
|
journal title
|
J Anal Toxicol (36/6)
|
|
ISSN electronic
|
1945-2403
|
|
pages
|
372-80
|
|
PubMed
|
22593567
|
|
DOI
|
10.1093/jat/bks048
|
additional links & downloads
