Comparison of extraction efficiencies and LC-MS-MS matrix effects using LLE and SPE methods for 19 antipsychotics in human blood
Eva Saar, Dimitri Gerostamoulos, Olaf H Drummer & Jochen Beyer
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abstract
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Antipsychotic drugs are frequently associated with sudden death
investigations. Detection of these drugs is necessary to establish
their use and possible contribution to the death. LC-MS(MS) methods
are common; however accurate and precise quantification is assured
by using validated methods. This study compared extraction
efficiency and matrix effects using common liquid-liquid and
solid-phase extraction procedures in both ante-mortem and
post-mortem specimen using LC-MS-MS. Extraction efficiencies and
matrix effects were determined in five different blank blood
specimens of each blood type. The samples were extracted using a
number of different liquid-liquid extraction methods and compared
with a standard mixed-mode solid-phase extraction method. Matrix
effects were determined using a post-extraction addition
approach-the blank blood specimens were extracted as described above
and the extracts were reconstituted in mobile phase containing a
known amount of analytes. The extraction comparison of ante-mortem
and post-mortem blood showed considerable differences, in particular
the extraction efficiency was quite different between ante-mortem
and post-mortem blood. Quantitative methods used for determination
of antipsychotic drugs in post-mortem blood should establish that
there are no differences in extraction efficiency and matrix
effects, particularly if using ante-mortem blood as calibrator.
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citation
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Saar E, Gerostamoulos D, Drummer O H, Beyer J. Comparison of
extraction efficiencies and LC-MS-MS matrix effects using LLE and
SPE methods for 19 antipsychotics in human blood. Anal Bioanal Chem
2008; 393:727-34.
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type
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journal paper/review (English)
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date of publishing
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11-11-2008
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journal title
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Anal Bioanal Chem (393/2)
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ISSN electronic
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1618-2650
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pages
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727-34
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PubMed
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19002441
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DOI
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10.1007/s00216-008-2498-6
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