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Upregulation of alpha-skeletal muscle actin and myosin heavy polypeptide gene products in degenerating rotator cuff muscles

Bruno Fuchs, Matthias Zumstein, Felix Regenfelder, Patrick Steinmann, Thomas Fuchs, Knut Husmann, Jens Hellermann, Bernhard Jost, Jürg Hodler, Walter Born & C Gerber

abstract Impaired function of shoulder muscles, resulting from rotator cuff tears, is associated with abnormal deposition of fat in muscle tissue, but corresponding cellular and molecular mechanisms, likely reflected by altered gene expression profiles, are largely unknown. Here, an analysis of muscle gene expression was carried out by semiquantitative RT-PCR in total RNA extracts of supraspinatus biopsies collected from 60 patients prior to shoulder surgery. A significant increase of alpha-skeletal muscle actin (p = 0.0115) and of myosin heavy polypeptide 1 (p = 0.0147) gene transcripts was observed in parallel with progressive fat deposition in the muscle, assessed on parasagittal T1-weighted turbo-spin-echo magnetic resonance images according to Goutallier. Upregulation of alpha-skeletal muscle actin and of myosin heavy polypeptide-1 has been reported to be associated with increased muscle tissue metabolism and oxidative stress. The findings of the present study, therefore, challenge the hypothesis that increased fat deposition in rotator cuff muscle after injury reflects muscle degeneration.
   
citation Fuchs B, Zumstein M, Regenfelder F, Steinmann P, Fuchs T, Husmann K, Hellermann J, Jost B, Hodler J, Born W, Gerber C. Upregulation of alpha-skeletal muscle actin and myosin heavy polypeptide gene products in degenerating rotator cuff muscles. J Orthop Res 2008; 26:1007-11.
   
type journal paper/review (English)
date of publishing 7-2008
journal title J Orthop Res (26/7)
ISSN electronic 1554-527X
pages 1007-11
PubMed 18327800
DOI 10.1002/jor.20577