1H-MRS in spinal cord injury: acute and chronic metabolite alterations in rat brain and lumbar spinal cord
Matthias Erschbamer, Johanna Oberg, Eric Westman, Rouslan Sitnikov, Lars Olson & Christian Spenger
abstract
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A variety of tests of sensorimotor function are used to characterize
outcome after experimental spinal cord injury (SCI). These tests
typically do not provide information about chemical and metabolic
processes in the injured CNS. Here, we used (1) H-magnetic resonance
spectroscopy (MRS) to monitor long-term and short-term chemical
changes in the CNS in vivo following SCI. The investigated areas
were cortex, thalamus/striatum and the spinal cord distal to injury.
In cortex, glutamate (Glu) decreased 1 day after SCI and slowly
returned towards normal levels. The combined glutamine (Gln) and Glu
signal was similarly decreased in cortex, but increased in the
distal spinal cord, suggesting opposite changes of the Glu/Gln
metabolites in cortex and distal spinal cord. In lumbar spinal cord,
a marked increase of myo-inositol was found 3 days, 14 days and 4
months after SCI. Changes in metabolite concentrations in the spinal
cord were also found for choline and N-acetylaspartate. No
significant changes in metabolite concentrations were found in
thalamus/striatum. Multivariate data analysis allowed separation
between rats with SCI and controls for spectra acquired in cortex
and spinal cord, but not in thalamus/striatum. Our findings suggest
MRS could become a helpful tool to monitor spatial and temporal
alterations of metabolic conditions in vivo in the brain and spinal
cord after SCI. We provide evidence for dynamic temporal changes at
both ends of the neuraxis, cortex cerebri and distal spinal cord,
while deep brain areas appear less affected.
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citation
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Erschbamer M, Oberg J, Westman E, Sitnikov R, Olson L, Spenger C.
1H-MRS in spinal cord injury: acute and chronic metabolite
alterations in rat brain and lumbar spinal cord. Eur J Neurosci
2011; 33:678-88.
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type
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journal paper/review (English)
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date of publishing
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20-01-2011
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journal title
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Eur J Neurosci (33/4)
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ISSN electronic
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1460-9568
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pages
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678-88
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PubMed
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21251091
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DOI
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10.1111/j.1460-9568.2010.07562.x
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